Echinacoside inhibited cardiomyocyte apoptosis and improved heart function in heart failure rats induced by isoproterenol via suppressing nadph/ros/atf6/chop associated endoplasmic reticulum stress

Abstract Funding Acknowledgements Type of funding sources: Other. Main source(s): Shaanxi Provincial Key Research and Development Projects (2019SF-218) Background Endoplasmic reticulum (ER) stress played an essential role in the development progression HF due to it could induce cardiomyocyte apoptosis, ATF6/CHOP was regulated by NADPH ROS crucial pathway link ER apoptosis HF. Our previous study indicated that ECH reversed cardiac remodeling improves heart function, but underlying mechanisms are still unclear. Objectives To investigate effect on endoplasmic mechanisms. Methods In vitro, we cultured AC-16 cells induced cell damage ISO, treated ECH, biomarkers (GRP78, ATF6α, IRE1α, PERK) were detected. vivo, rat model ISO indexes NADPH/ROS/ATF6/CHOP measured. Results confirmed inhibited apoptosis. demonstrated improved significantly decreased GRP78, PERK, down-regulated pathway. Conclusions function via suppressing associated stress.